| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SUN5 recombinant protein (Position: E16-K341) was used as the immunogen for the SUN5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SUN5 Antibody / Sad1 and UNC84 domain-containing protein 5 is a anti-SUN5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SUN5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, SUN5 antibody identifies a 379-amino-acid protein localized to the inner nuclear membrane, where it interacts with outer membrane proteins such as Nesprins through its conserved SUN domain. SUN5 is particularly enriched in spermatids, where it mediates head-to-tail coupling during sperm maturation. Loss of SUN5 disrupts this linkage, leading to acephalic spermatozoa and infertility.
The SUN5 gene is located on chromosome 20q11.22 and is predominantly expressed in testis, with lower expression in other tissues. Its testis-specific expression pattern underscores its specialized function in spermiogenesis. SUN5 contributes to nuclear shaping, centrosome attachment, and flagellar organization through mechanical coupling between the nucleus and cytoskeleton.
Pathologically, mutations in SUN5 are associated with acephalic spermatozoa syndrome and male infertility due to defective sperm head-tail junction formation. Research using SUN5 antibody supports studies in reproductive biology, nuclear envelope architecture, and cytoskeletal interaction mechanisms.
SUN5 antibody is validated for use in western blotting, immunofluorescence, and immunohistochemistry to study LINC complex components and spermatid structure.
Structurally, SUN5 contains a coiled-coil region and a conserved C-terminal SUN domain that mediates interaction with KASH domain proteins across the nuclear envelope. This antibody helps elucidate SUN5's molecular function in nuclear-cytoskeletal attachment and fertility-related disorders.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
