| Field | Specification |
|---|---|
| Mfr No | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | horse-radish peroxidase and its conjugates |
| Shipping | |
| Species | |
| Storage |
Overview
For HRP determination in ELISA, Southern Blot, Western Blot. The assay uses Luminescence for signal readout. Compatible sample input includes horse-radish peroxidase and its conjugates. Typical stated assay timing is 10 min.
Key elements and design rationale
- Readout format: Luminescence supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes horse-radish peroxidase and its conjugates, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of Atto- to low femtomolar for interpreting low-signal samples.
- Feature emphasis: Single reagent: Single, stable, and ready to use.
Additional feature notes highlight High sensitivity: Detection of HRP in the atto- to low femtogram range. Available format information for this listing includes 200 Tests in 96-well plate (800 Tests in 384-well plate).
Biological background
This product is centered on measurement of superlight atto hrp within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
PEROXIDASES(or peroxide reductases, EC number 1.11.1.x) are a large group of enzymes that break up peroxides and play important roles in various biological processes. Horse Radish Peroxidase (HRP) is an enzyme commonly used for conjugation. One example is antibody-HRP conjugate that is frequently and widely used in immunoassays such as ELISA and Western blots. BioAssay Systems’ SuperLight™ Atto HRP Reagent provides an ultra-sensitive luminescent means for detecting HRP activity and its conjugates. The improved reagent rapidly reacts with peroxidase or the HRP in an HRP-conjugate, producing highly stable and intense luminescence for maximum detection of the peroxidase activity in solution assays.
Detection method
Luminescence.
Detection limit and analytical sensitivity
Reported detection limit: Atto- to low femtomolar.
Procedures and timing
Stated procedure or timing information: 10 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
- Matched standards, blanks, and replicate wells are typically used to improve interpretability across batches and sample matrices.
Common research applications
- Prepare control-treated samples for paired use with compatible assay-kit plates.
- Benchmark assay response against defined control conditions in replicate wells.
- Optimize reagent concentration and incubation windows for plate-based comparisons.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
What are the applications of this reagent?
This ultra-sensitive reagent is used to detect peroxidase activity (e.g. horse-radish peroxidase) and its conjugates in ELISA or Western blots.
What equipment should I use?
You can use any luminometer, such as Molecular Devices, Perkin Elmer readers.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
