| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SUPT16H recombinant protein (Position: M1-E926) was used as the immunogen for the SUPT16H antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SUPT16H Antibody / Suppressor of Ty 16 is a anti-SUPT16H Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: SUPT16H
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
SUPT16H is encoded by the SUPT16H gene located on human chromosome 14q11.2. The protein is approximately 120 kilodaltons and consists of an N-terminal dimerization domain, a middle domain that binds histones H2A-H2B, and a C-terminal acidic region responsible for interactions with RNA polymerase II and other transcriptional regulators. Through its role in nucleosome disassembly and reassembly, SUPT16H maintains chromatin integrity during active transcription.
A SUPT16H antibody detects a 120 kilodalton band in western blot assays and demonstrates strong nuclear localization by immunofluorescence. FACT complex activity is essential for the passage of polymerases through chromatin and the preservation of epigenetic marks. SUPT16H regulates promoter accessibility and prevents inappropriate histone eviction, balancing gene activation with chromatin stability.
Altered SUPT16H function affects transcription elongation, replication stress response, and genome maintenance. Viral proteins such as HIV Tat exploit FACT to facilitate viral transcription, while suppression of SUPT16H reduces viral replication. Overexpression is observed in several cancers, where it supports rapid proliferation by maintaining chromatin fluidity and preventing DNA damage-induced senescence.
Because of its pivotal role in chromatin dynamics, SUPT16H serves as a potential target for cancer therapy and antiviral drug development.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
