| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SUPT20H recombinant protein (Position: M1-Q713) was used as the immunogen for the SUPT20H antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SUPT20H Antibody / Transcription factor SPT20 homolog is a anti-SUPT20H Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: SUPT20H
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, ELISA
Biological background
Structurally, SUPT20H contains conserved coiled-coil and low-complexity regions that facilitate interactions with chromatin-associated proteins, transcription factors, and the proteasome. It is a functional homolog of yeast SPT20, a core subunit of the SAGA (Spt-Ada-Gcn5 acetyltransferase) transcriptional coactivator complex that links histone acetylation to gene activation. In mammalian systems, SUPT20H contributes to nucleosome remodeling and transcriptional coactivation by coupling histone modification with recruitment of RNA polymerase II at promoter regions.
The SUPT20H antibody is widely used in epigenetics, gene regulation, and cell biology research to study transcriptional activation, chromatin remodeling, and stress adaptation. Western blot analysis detects a 100 kilodalton band corresponding to SUPT20H, while immunofluorescence reveals both nuclear and cytoplasmic staining, reflecting its multifunctional localization. This antibody enables researchers to investigate how chromatin dynamics and transcriptional machinery coordinate gene expression in response to cellular cues.
Beyond its nuclear role, SUPT20H interacts with the proteasome and heat shock proteins to regulate protein degradation and quality control under stress conditions. It assists in clearing misfolded proteins and maintaining cytoplasmic proteostasis. Dysregulation of SUPT20H expression or mutations affecting its protein-interaction motifs have been linked to altered transcriptional fidelity, stress sensitivity, and oncogenic transformation. The SUPT20H antibody provides a powerful tool for characterizing these processes at the molecular level.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
