| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SUSD2 recombinant protein (Position: D301-H593) was used as the immunogen for the SUSD2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SUSD2 Antibody / Sushi domain-containing protein 2 is a anti-SUSD2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: SUSD2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, SUSD2 antibody identifies a 822-amino-acid membrane protein that includes somatomedin B, AMOP, von Willebrand factor type D, and Sushi (CCP) domains. These motifs facilitate cell-cell and cell-matrix adhesion while modulating immune recognition and tumor microenvironment interactions. SUSD2 supports epithelial integrity and participates in TGF-beta and Wnt signaling pathways that control cell differentiation and invasion.
The SUSD2 gene is located on chromosome 22q11.23 and is expressed in endothelial cells, epithelial tissues, and several cancer types. Its expression is elevated in breast, ovarian, and colorectal cancers, where it contributes to tumor growth, angiogenesis, and immune evasion. In normal physiology, SUSD2 is thought to support epithelial repair and maintain mucosal homeostasis.
Pathologically, overexpression of SUSD2 promotes tumor cell adhesion, migration, and immune suppression through interactions with Galectin-1 and other immunoregulatory factors. Conversely, in certain contexts, SUSD2 may enhance antitumor immune responses by facilitating macrophage activation. Research using SUSD2 antibody supports studies in cancer immunology, cell adhesion, and signal transduction.
SUSD2 antibody is validated for western blotting, immunohistochemistry, and flow cytometry to detect adhesion-related glycoproteins.
Structurally, Sushi domain-containing protein 2 features multiple modular domains connected by disulfide bonds, forming a flexible extracellular scaffold that mediates protein-protein interactions. The cytoplasmic tail contains potential phosphorylation sites that regulate receptor signaling and intracellular trafficking. This antibody enables investigation of SUSD2's functional role in adhesion, cancer progression, and immune modulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
