| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human C1orf26/SWT1 recombinant protein (Position: Q322-R889) was used as the immunogen for the SWT1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SWT1 Antibody / C1orf26 is a anti-SWT1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SWT1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Pre-mRNA-splicing factor SWT1 homolog localizes to nucleoplasmic and nucleolar regions where active splicing occurs. It contains conserved zinc-finger and endonuclease domains required for RNA substrate recognition and cleavage. The SWT1 antibody allows visualization of this nuclear factor, enabling the investigation of its roles in processing small nuclear RNAs (snRNAs) and small nucleolar RNAs (snoRNAs). Through these actions, SWT1 contributes to ribosome biogenesis and overall RNA quality assurance within the nucleus.
Loss or dysfunction of Pre-mRNA-splicing factor SWT1 homolog leads to accumulation of defective pre-mRNA and abnormal spliceosome assembly, disrupting normal gene expression. The SWT1 antibody aids in identifying changes in SWT1 expression or localization in experimental systems subjected to transcriptional stress or RNA splicing inhibition. Studies indicate that this factor participates in safeguarding the transcriptome against RNA processing defects that can trigger cellular stress responses.
Beyond RNA metabolism, SWT1 may interact with other nuclear RNA decay enzymes, coordinating pathways that remove splicing intermediates and cryptic transcripts. The SWT1 antibody supports mechanistic studies exploring these complexes, helping define how nuclear RNA degradation interfaces with splicing surveillance. Its conservation from yeast to humans highlights an evolutionarily stable role in RNA integrity maintenance.
The SWT1 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing strong nuclear staining consistent with RNA processing activity.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
