| Field | Specification |
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| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human SYF2 was used as the immunogen for the SYF2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SYF2 Antibody / Pre-mRNA splicing factor SYF2 is a anti-SYF2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: SYF2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS
Biological background
SYF2 interacts with PRPF19, CDC5L, and other components of the spliceosome-associated NTC complex to facilitate transition between spliceosome conformations. It also coordinates splicing with transcription by associating with RNA polymerase II elongation complexes. In addition to its role in RNA processing, SYF2 is involved in G1/S phase progression, likely through regulation of splicing of genes involved in DNA replication and repair. Depletion of SYF2 disrupts normal cell cycle progression, underscoring its role in proliferative control.
The SYF2 antibody is used in RNA biology, cell cycle, and genomics research to study spliceosome assembly and mRNA maturation. Western blot analysis identifies a 24 kilodalton band corresponding to SYF2, and immunofluorescence demonstrates nuclear localization consistent with its splicing function. This antibody enables monitoring of SYF2 expression and localization in studies of transcriptional regulation and RNA processing.
In cancer research, SYF2 has emerged as a proliferation-associated factor, with overexpression reported in breast, lung, and colorectal cancers. It may contribute to tumor progression by promoting efficient splicing of growth-related transcripts. The SYF2 antibody provides a reliable tool for exploring splicing factor regulation, cell cycle control, and their intersections in disease mechanisms.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
