| Field | Specification |
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| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Nesprin3 was used as the immunogen for the SYNE3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SYNE3 Antibody / Nesprin 3 is a anti-SYNE3 Rabbit antibody Recombinant Rabbit Monoclonal clone 30S37 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: SYNE3
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30S37, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
The LINC complex serves as a conduit for mechanical signals transmitted between the cytoplasm and the nucleus. By anchoring the nuclear envelope to cytoskeletal elements, Nesprin3 helps stabilize nuclear shape and position during migration, mitosis, and differentiation. When detected with SYNE3 antibody, changes in its expression or distribution can be correlated with altered mechanosensing and transcriptional regulation. These processes are essential for proper tissue development and for cellular responses to physical stress, making the study of SYNE3 protein highly relevant to mechanobiology.
Dysregulation of nuclear envelope proteins contributes to a variety of diseases, including muscular dystrophies, cardiomyopathies, and neurological disorders. Although more attention has historically focused on Nesprin1 and Nesprin2, studies suggest that Nesprin 3 also participates in these pathways by weakening nuclear anchorage when perturbed. SYNE3 antibody enables detection of protein alterations in both healthy and diseased tissue, providing a means to assess its contribution to pathogenesis. Evidence has also linked SYNE3 to cancer progression, where nuclear deformability affects metastatic cell migration through dense tissue matrices.
SYNE3 antibody is widely used in western blotting, immunofluorescence, and immunohistochemistry. In western blotting, it detects protein levels across tissue lysates, while immunofluorescence confirms its nuclear envelope localization in cultured cells. Immunohistochemistry highlights expression patterns in tissues such as muscle, skin, and epithelium, reflecting its role in nuclear architecture. Functional studies using SYNE3 antibody in knockdown and rescue experiments have demonstrated how loss of the protein disrupts nuclear positioning and cell motility, reinforcing its role in structural and signaling networks.
By providing validated SYNE3 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
