| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SYNJ2BP recombinant protein (Position: D32-L145) was used as the immunogen for the SYNJ2BP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SYNJ2BP Antibody / Synaptojanin-2-binding protein is a anti-SYNJ2BP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SYNJ2BP
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
SYNJ2BP is encoded by the SYNJ2BP gene on human chromosome 14q22.1. The protein is approximately 118 amino acids in length and contains a PDZ-binding motif that mediates interactions with synaptojanin-2 and other cytoplasmic adaptors. It localizes to the outer mitochondrial membrane, where it plays a role in tethering mitochondria to the endoplasmic reticulum and modulating calcium and lipid exchange between the two organelles.
The SYNJ2BP antibody detects a 13 kilodalton band by western blot and exhibits mitochondrial and perinuclear staining patterns under confocal microscopy. SYNJ2BP interacts with the PDZ domain of SYNJ2, which links phosphoinositide metabolism to mitochondrial signaling. By maintaining proper mitochondrial-endoplasmic reticulum contact sites, SYNJ2BP supports cellular homeostasis and regulates apoptotic sensitivity under metabolic stress.
Functionally, SYNJ2BP contributes to the stabilization of mRNAs on the mitochondrial surface and may assist in local translation of mitochondrial proteins. Loss of SYNJ2BP disrupts organelle coupling and leads to mitochondrial fragmentation, altered calcium signaling, and reduced energy efficiency. In cancer and metabolic disorders, SYNJ2BP expression correlates with altered mitochondrial dynamics and oxidative phosphorylation capacity.
Beyond its role in organelle tethering, SYNJ2BP participates in endothelial cell migration and angiogenesis by coordinating signal transduction from cell surface receptors to mitochondria. This function connects metabolic status with cell motility and vascular remodeling. Because of its position at the interface of metabolism, trafficking, and signaling, SYNJ2BP is a valuable model for studying mitochondrial communication and adaptation to stress.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
