| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SYNPO2L recombinant protein (Position: H66-E923) was used as the immunogen for the SYNPO2L antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SYNPO2L Antibody / Synaptopodin 2-like protein is a anti-SYNPO2L Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat.
Key elements and design rationale
- Target: SYNPO2L
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Structurally, SYNPO2L contains actin-binding domains and proline-rich motifs that mediate interaction with alpha-actinin and other cytoskeletal proteins. It helps anchor signaling molecules at Z-discs, coordinating mechanical and biochemical responses during muscle contraction and adaptation. Through its role in actin filament organization, SYNPO2L contributes to cardiomyocyte differentiation and maintenance of sarcomere integrity.
The SYNPO2L antibody is widely used in cardiovascular and muscle research to study cytoskeletal architecture, myofibril assembly, and Z-disc organization. Western blot analysis detects a 110 kilodalton band corresponding to SYNPO2L, while immunofluorescence shows striated staining patterns characteristic of sarcomeric proteins. This antibody is valuable for investigating the molecular mechanisms of cardiac development, contractile regulation, and myopathy pathogenesis.
Genetic variants in SYNPO2L are associated with atrial fibrillation and cardiomyopathy, suggesting its importance in cardiac conduction and mechanical stability. Altered expression of SYNPO2L may also affect stress fiber organization in non-muscle cells, influencing cell migration and adhesion. The SYNPO2L antibody supports studies of muscle structure, cytoskeletal signaling, and heart disease mechanisms.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
