| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Synaptotagmin-10/SYT10 recombinant protein (Position: M1-D363) was used as the immunogen for the SYT10 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SYT10 Antibody / Synaptotagmin 10 is a anti-SYT10 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cell membrane, vesicles.
Key elements and design rationale
- Target: SYT10
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ELISA
Biological background
Functionally, SYT10 antibody identifies a 565-amino-acid protein that plays a role in the regulation of insulin secretion and neuronal exocytosis. SYT10 is unique among synaptotagmins because it is expressed in both neuronal and non-neuronal tissues, including the pancreas and pituitary gland. It is associated with the release of insulin-like growth factor 1 (IGF-1) and other neuropeptides in a calcium-dependent manner. Through its C2A and C2B domains, SYT10 mediates the calcium-triggered docking and fusion of secretory vesicles, ensuring precise timing of exocytosis following intracellular signaling events.
The SYT10 antibody is used to study secretory pathways, vesicular transport, and neuronal communication. In neurons, SYT10 localizes to presynaptic membranes and regulates neurotransmitter release by acting as a Ca2+-sensor for synaptic vesicle fusion. In endocrine cells, it supports hormone secretion by coupling calcium signaling to vesicular exocytosis. Expression of SYT10 is enriched in specific brain regions such as the cortex and cerebellum, reflecting specialized regulatory roles in neurotransmission.
Mutations or dysregulation of SYT10 have been associated with altered synaptic signaling and insulin secretion. The SYT10 gene is located on chromosome 12q21.32 and encodes a protein with two conserved C2 domains responsible for calcium and phospholipid binding. Unlike some synaptotagmins that promote rapid synchronous release, SYT10 is implicated in sustained and asynchronous release events. Research indicates that SYT10 may also participate in neurotrophic signaling and membrane repair processes.
SYT10 antibody applications include western blotting, immunocytochemistry, and confocal microscopy to examine vesicle trafficking, synaptic plasticity, and calcium-triggered secretion. Its use extends to neuroendocrine and pancreatic models where SYT10 mediates regulated exocytosis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
