| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Synaptotagmin-12/SYT12 recombinant protein (Position: Q63-R410) was used as the immunogen for the SYT12 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SYT12 Antibody / Synaptotagmin 12 is a anti-SYT12 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SYT12
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
SYT12 is encoded by the SYT12 gene located on human chromosome 11q12.1. The protein is approximately 240 amino acids long and contains two C2-like domains typical of the synaptotagmin family. However, substitutions in key residues render these domains calcium-insensitive, distinguishing SYT12 as a regulator of synaptic machinery rather than a classical calcium sensor. SYT12 localizes predominantly to presynaptic terminals and associates with SNARE complex components that drive vesicle fusion.
An SYT12 antibody detects a 28 kilodalton band by western blot and reveals punctate staining at presynaptic sites under confocal microscopy. SYT12 interacts with SNAP25 and syntaxin, modulating the assembly of the SNARE complex and influencing vesicle priming efficiency. Experimental silencing of SYT12 in neurons decreases spontaneous and evoked neurotransmitter release, indicating its role in maintaining basal synaptic transmission.
Beyond synaptic function, SYT12 participates in hormone secretion and calcium-independent vesicle trafficking in endocrine cells. Mutations in SYT12 have been linked to neurological and psychiatric conditions, including epilepsy and movement disorders. In cancer research, SYT12 overexpression correlates with poor prognosis in lung and colorectal cancers, where it enhances tumor cell migration through altered vesicle dynamics.
SYT12 exemplifies the regulatory diversity of synaptotagmin family proteins, balancing vesicle fusion without directly sensing calcium.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
