| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SYT17 recombinant protein (Position: R24-K304) was used as the immunogen for the SYT17 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SYT17 Antibody / Synaptotagmin 17 is a anti-SYT17 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: SYT17
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, ELISA
Biological background
Unlike canonical synaptotagmins that function in neurotransmitter release, SYT17 is primarily expressed in non-synaptic regions of the brain, kidney, and liver, where it regulates membrane trafficking between the endoplasmic reticulum and Golgi apparatus. It may modulate calcium-sensitive steps in vesicle fusion and contribute to secretory pathway organization. In neurons, SYT17 expression has been linked to dendritic cargo transport and neurite extension, implicating it in intracellular trafficking rather than synaptic vesicle exocytosis. Loss or mutation of SYT17 alters intracellular transport dynamics, potentially affecting neural development and metabolic processes.
The SYT17 antibody is used in neuroscience and cell biology to detect Synaptotagmin-17 expression and subcellular localization. Western blot analysis typically identifies a ~50 kilodalton band, while immunofluorescence reveals punctate cytoplasmic staining consistent with endomembrane localization. In neuronal cultures, SYT17 co-localizes with markers of the Golgi network and early endosomes, indicating its role in vesicular transport. The antibody aids in studying membrane trafficking, calcium signaling, and synaptotagmin family diversification.
Functionally, SYT17 binds phospholipids in a calcium-dependent manner and may participate in regulating the SNARE machinery under specific conditions. It also interacts with proteins involved in vesicle tethering and sorting. Dysregulated SYT17 expression has been reported in certain neurodegenerative disorders and cancers, though its exact pathogenic roles remain under investigation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
