| Field | Specification |
|---|---|
| Mfr No | |
| Product Format | Frozen |
| Product Type | |
| Shipping | |
| Storage |
Overview
abm's primary human Natural Killer (NK) cells (CD3⁻CD56⁺) are freshly isolated or cryopreserved from healthy donor peripheral blood using advanced immunomagnetic selection techniques. These cells retain robust viability, cytolytic activity, and cytokine production potential, making them ideal for immunological studies, cell-based therapy development, and high-throughput drug screening. The product is supplied as frozen cells.
Key elements and design rationale
- Biological source: Human (H. sapiens)
- Tissue origin: Peripheral Blood
- Growth properties: Suspension, round
- Format: Frozen
- Biosafety level: II
- Culture context: PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. PrimGrow NK Xpan™ Medium Kit (TM183) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
Natural killer cells provide innate immune surveillance and are widely used to study activation-state changes, cytotoxic response programs, and cytokine-driven functional modulation.
Research relevance and current trends
- Primary NK-cell studies commonly evaluate activation, phenotype, and target-cell response under defined cues.
- Investigators examine donor variability and subset composition in functional immune assays.
- Co-culture systems are used to probe reciprocal signaling with tumor, stromal, or antigen-presenting cells.
Common research applications
- Assess activation markers, cytokine responses, or cytotoxicity-associated readouts.
- Use in co-culture systems that require primary innate immune effector cells.
- Profile donor-dependent functional changes following perturbation.
Product-specific data supplied for this listing
- Growth Conditions: PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. PrimGrow NK Xpan™ Medium Kit (TM183) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
- Warranty: abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”.
- Disclaimer: 1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at orders@biohippo.com.
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
- Simply add fresh complete media directly to the culture. Do not allow cell density to exceed 1x10⁶ cells/ml.
- Alternatively, replace complete growth media by centrifugation and re-suspend the cell pellet in fresh complete media, and add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
