| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
T4 Endonuclease V, also known as T4 PDG (Pyrimidine Dimer Glycosylase), is a bifunctional DNA glycosylase that combines the activities of DNA N-glycosylase and AP lyase. This enzyme is capable of recognizing and binding to cis-syn cyclobutane pyrimidine dimers, which are induced by ultraviolet radiation. It then cleaves the glycosidic bond at the 5' end of the pyrimidine dimers, releasing them and leaving an AP site. Subsequently, it utilizes its endonuclease activity to cleave the phosphodiester bond at the AP site, creating a one-nucleotide gap in the DNA strand.
Applications
DNA damage studies
Single cell gel electrophoresis (comet assay)
Specifications
|
Source |
T4 Phage |
|
Molecular Weight |
16.1 KD |
|
Concentration |
1 U/μL |
|
Unit Definition |
One unit is defined as the amount of enzyme that catalyzes the conversion of 0.5 µg of UV irradiated supercoiled pUC19 DNA to > 95% nicked plasmid in a total reaction volume of 20 µL in 30 minutes at 37°C. Nicking is assessed by agarose gel electrophoresis. Irradiated plasmid contains an average of 3-5 pyrimidine dimers |
Glycerol Content |
Contains Glycerol |
Components
|
Components No. |
Name |
14542ES76 |
14542ES80 |
|
14542-A |
T4 Endonuclease V(T4 PDG)(1 U/μL) |
500 μL |
1 mL |
|
14542-B |
10 x T4 Endonuclease V(T4 PDG) Buffer |
1 mL |
2 mL |
Shipping and Storage
This product should be stored at-25~-15℃ for 1 years.
Documents:
Safety Data Sheet
Manuals
14542_Manual _Ver.EN20250121.pdf
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
DNA damage studies Single cell gel electrophoresis (comet assay). Always verify compatibility with your specific template, buffer, and downstream workflow.
One unit (U) is defined as the amount of enzyme that degrades 1 µg of DNA or RNA substrate to acid-soluble form in 30 min at 37°C under defined buffer conditions.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Nuclease activity typically requires divalent metal ion cofactors (Mg²⁺ or Mn²⁺ at 1–5 mM). Activity is inhibited by chelating agents such as EDTA (≥1 mM), high salt concentrations, and reducing agents such as DTT at elevated concentrations. Heat inactivation (65–75°C for 15 min) is effective for most nucleases, allowing removal of enzyme activity after digestion without column purification.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
