T4 Polynucleotide Kinase (10 U/μL)

Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.

Phosphorylation of the 5' ends of DNA or RNA for subsequent ligation Terminal labeling of DNA or RNA to serve as probes and for sequencing purposes 5' phosphorylation of mononucleotides with a phosphorylated 3' end to prepare pNp substrates for attachment to the 3' ends of DNA or RNA Labeling the 5' ends of oligonucleotides that possess a 3' phosphate group. Always verify compatibility with your specific template, buffer, and downstream workflow.

One unit (U) is defined as the amount of enzyme that catalyzes the phosphorylation or dephosphorylation of 1 nmol of substrate per minute at 37°C under standard buffer conditions.

This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of a γ-phosphate from ATP to the 5′-hydroxyl terminus of DNA or RNA, enabling ligation of blunt-end or cohesive-end fragments lacking 5′ phosphates. Shrimp Alkaline Phosphatase (SAP) removes 5′ and 3′ phosphate groups from DNA, RNA, and dNTPs, and is heat-inactivated at 65°C for 15 min—eliminating the need for column cleanup prior to downstream reactions.