| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TAF15 recombinant protein (Position: Y63-R395) was used as the immunogen for the TAF15 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TAF15 Antibody / TATA-binding protein-associated factor 2N is a anti-TAF15 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TAF15
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, TAF15 antibody identifies a 592-amino-acid nuclear protein characterized by RNA recognition motifs (RRMs), a low-complexity prion-like domain, and a glycine-rich C-terminus. TAF15 interacts with the TFIID complex, serving as a coactivator in transcription initiation by RNA polymerase II. It also binds nascent transcripts, coordinating RNA processing and export. Through its prion-like domain, TAF15 can assemble into reversible RNA-protein granules under stress, supporting mRNA storage and translational regulation.
The TAF15 gene is located on chromosome 17q11.2 and is ubiquitously expressed, with particularly high levels in neurons and other transcriptionally active cells. TAF15 acts at the interface of chromatin remodeling and RNA metabolism, modulating transcriptional output and transcript stability. It regulates genes involved in differentiation, cell cycle control, and stress response, serving as a key integrator of nuclear RNA metabolism.
Clinically, TAF15 has been implicated in oncogenic translocations and neurodegenerative disorders. Chromosomal rearrangements involving TAF15 generate fusion oncoproteins such as TAF15-NR4A3 and TAF15-CREB1, which act as aberrant transcriptional activators in sarcomas and leukemia. In the nervous system, abnormal aggregation of TAF15 has been linked to amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), where it contributes to RNA granule pathology and cytoplasmic mislocalization.
TAF15 antibody is widely used in RNA biology, transcriptional regulation, and neurodegeneration research. It is suitable for immunoblotting, immunofluorescence, and chromatin immunoprecipitation to detect TAF15 localization and activity. This antibody supports studies of RNA processing, stress granule formation, and oncogenic fusions. In translational research, TAF15 detection aids in characterizing RNA-protein interactions and gene expression networks underlying disease.
Structurally, TAF15 combines RNA-binding RRMs with intrinsically disordered regions that mediate multivalent interactions with RNA and chromatin-associated proteins.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
