| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human TAF3 was used as the immunogen for the TAF3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TAF3 Antibody / TATA-box binding protein associated factor 3 is a anti-TAF3 Rabbit antibody Recombinant Rabbit Monoclonal clone 30T12 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human.
Key elements and design rationale
- Target: TAF3
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30T12, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF
Biological background
TATA-box binding protein associated factor 3 interacts directly with TATA-box binding protein and other TAF subunits to stabilize the transcription preinitiation complex at promoter regions. Research using TAF3 antibody has shown that it also recognizes histone modifications, particularly H3K4me3, through its plant homeodomain finger. This allows TAF3 to couple chromatin state with transcription initiation, providing a mechanism for epigenetic regulation of gene expression.
Studies with TAF3 antibody have revealed that TATA-box binding protein associated factor 3 plays critical roles during development and differentiation. It is required for myogenic differentiation and early embryonic gene expression programs. Loss of TAF3 disrupts promoter recognition and impairs transcriptional activation, highlighting its central function in developmental biology.
Dysregulation of TATA-box binding protein associated factor 3 has been linked to disease. Research using TAF3 antibody has shown that altered expression contributes to cancer by modifying transcriptional programs. Because of its ability to read epigenetic marks, TAF3 is a key regulator of gene networks that govern proliferation and differentiation. These findings underscore its importance in both normal physiology and pathology.
TAF3 antibody is widely applied in chromatin immunoprecipitation, western blotting, and immunohistochemistry. Chromatin immunoprecipitation maps TAF3 binding at promoters, western blotting detects full-length and isoform expression, and immunohistochemistry localizes TAF3 in nuclei across tissues. These applications make TAF3 antibody indispensable for transcription research.
By providing validated TAF3 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
