| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TAF3 recombinant protein (Position: M1-E612) was used as the immunogen for the TAF3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TAF3 Antibody / Transcription initiation factor TFIID subunit 3 is a anti-TAF3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear.
Key elements and design rationale
- Target: TAF3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, FACS, ELISA
Biological background
Functionally, TAF3 antibody identifies a 929-amino-acid nuclear protein that contains a PHD finger domain recognizing histone H3K4me3, a chromatin mark associated with active transcription. TAF3 interacts with the TATA-binding protein (TBP) and other TAFs to form the pre-initiation complex, directing RNA polymerase II to target promoters. It also mediates enhancer-promoter looping during gene activation.
The TAF3 gene is located on chromosome 10q22.1 and is broadly expressed in embryonic and differentiated tissues. In muscle and hematopoietic cells, TAF3 cooperates with transcription factors like MyoD and C/EBP to regulate lineage-specific gene expression. Through its histone mark recognition, TAF3 connects epigenetic signals to transcriptional initiation.
Pathologically, dysregulation of TAF3 has been linked to developmental disorders and cancers. Altered TAF3 expression affects chromatin accessibility and transcriptional fidelity, contributing to tumor progression or differentiation defects. Research using TAF3 antibody supports studies in transcription regulation, epigenetics, and cell fate determination.
TAF3 antibody is validated for western blotting, immunofluorescence, and chromatin immunoprecipitation to detect transcriptional machinery components.
Structurally, Transcription initiation factor TFIID subunit 3 contains a plant homeodomain (PHD) that binds methylated histones, enabling crosstalk between chromatin and transcriptional complexes. This antibody allows detailed analysis of TAF3's role in gene regulation and epigenetic control.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
