| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TAF6 recombinant protein (Position: S16-R479) was used as the immunogen for the TAF6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TAF6 Antibody / TATA-box binding protein-associated factor 6 is a anti-TAF6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: TAF6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, IP, ELISA
Biological background
TAF6 is an integral structural and functional component of TFIID, contributing to promoter recognition, nucleosome remodeling, and transcription start site selection. The TFIID complex serves as a scaffold for preinitiation complex formation, interacting with transcriptional regulators, general transcription factors, and coactivators. TAF6 participates in DNA binding and mediates protein-protein interactions with other TAF subunits, including TAF9 and TAF12, forming submodules that stabilize TFIID assembly and promoter binding.
The TAF6 antibody identifies a protein of approximately 68 kDa encoded by the TAF6 gene on chromosome 7q22.3. Multiple isoforms of TAF6 are generated through alternative splicing, including the pro-apoptotic TAF6? variant, which lacks a short exon and promotes programmed cell death independently of p53 signaling. The balance between canonical TAF6 and TAF6? isoforms influences cellular responses to stress and apoptosis, highlighting the protein's dual role in both transcriptional regulation and cell survival pathways.
Functionally, TAF6 contributes to basal and regulated transcription by integrating signals from transcriptional activators and chromatin modifiers. It participates in the regulation of genes involved in cell cycle progression, DNA repair, and differentiation. TAF6 dysfunction or altered expression has been associated with impaired gene regulation and has been investigated in the context of cancer and neurodegenerative disorders, where transcriptional control is disrupted.
TAF6 antibody is useful for applications such as immunoblotting, immunohistochemistry, and immunofluorescence to study transcription complex composition, promoter occupancy, and RNA polymerase II recruitment. It enables detection of TAF6 in nuclear extracts, cultured cells, and tissue samples, supporting research into transcriptional regulation and stress-induced gene expression.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
