| Field | Specification |
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| Mfr No | |
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| Host | |
| Immunogen | A synthesized peptide derived from human TAGAP was used as the immunogen for the TAGAP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TAGAP Antibody / T cell activation Rho GTPase activating protein is a anti-TAGAP Rabbit antibody Recombinant Rabbit Monoclonal clone 30T40 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TAGAP
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30T40, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IP
Biological background
T cell activation Rho GTPase activating protein functions as a negative regulator of Rho GTPases by stimulating their intrinsic GTPase activity, converting active GTP bound forms into inactive GDP bound forms. Research using TAGAP antibody has shown that this regulation influences T cell activation thresholds and cytoskeletal reorganization during immune responses. By fine tuning Rho signaling, TAGAP modulates T cell migration, adhesion, and synapse formation, processes that are critical for adaptive immunity.
Genome wide association studies have identified TAGAP as a susceptibility locus for several autoimmune diseases, including multiple sclerosis, type 1 diabetes, rheumatoid arthritis, and celiac disease. Studies with TAGAP antibody have revealed that altered expression or polymorphisms in TAGAP influence T cell function, predisposing individuals to dysregulated immune responses. These findings highlight TAGAP as a genetic link between cytoskeletal regulation and autoimmunity.
Beyond its role in T cells, TAGAP is expressed in other immune lineages such as dendritic cells and macrophages. Research using TAGAP antibody has demonstrated roles in innate immune signaling, where TAGAP contributes to pattern recognition receptor pathways and cytokine production. This expands its relevance beyond adaptive immunity to innate immune regulation.
TAGAP antibody is widely used in western blotting, immunohistochemistry, and flow cytometry. Western blotting demonstrates inducible expression after immune stimulation, immunohistochemistry shows localization in lymphoid tissues, and flow cytometry quantifies expression across immune subsets. These applications make TAGAP antibody a versatile tool in immunology research.
By supplying validated TAGAP antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
