| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TAOK1 recombinant protein (Position: Q424-R884) was used as the immunogen for the TAOK1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TAOK1 Antibody / TAO kinase 1 is a anti-TAOK1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TAOK1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IP, FACS, ELISA
Biological background
Functionally, TAOK1 antibody identifies a 100 kDa enzyme that phosphorylates and activates MAP2Ks such as MKK3 and MKK6, leading to downstream activation of p38 MAPK. TAOK1 also modulates the cytoskeleton by interacting with microtubule-associated proteins, including MARK kinases. This regulation is critical for neuronal polarity, axon specification, and dendrite formation. During mitosis, TAOK1 contributes to spindle orientation and chromosome alignment, linking stress signaling with cell-cycle progression.
The TAOK1 gene is located on chromosome 17q11.2 and encodes a multi-domain kinase composed of an N-terminal catalytic domain and a C-terminal regulatory region. It functions as both a signaling hub and structural organizer by localizing to centrosomes, Golgi membranes, and the cytoplasmic microtubule network. Autophosphorylation and stress-induced activation enhance TAOK1 activity, enabling rapid response to environmental and mechanical stimuli.
TAOK1 has gained prominence for its role in neurodevelopment. Mutations or dysregulation of TAOK1 are associated with neurodevelopmental disorders, including autism spectrum disorder and intellectual disability. Experimental evidence shows that TAOK1 depletion impairs axonal growth, disrupts neuronal morphology, and alters synaptic signaling. In non-neuronal cells, TAOK1 mediates apoptotic signaling through activation of p38 and JNK under oxidative or osmotic stress conditions.
TAOK1 antibody is a versatile reagent for analyzing kinase signaling, cytoskeletal organization, and stress-induced apoptosis. It is commonly used in immunoblotting, immunofluorescence, and kinase activity assays to evaluate TAOK1 expression and activation. In cancer research, TAOK1 contributes to epithelial-to-mesenchymal transition (EMT) and metastasis, while in neurons, it supports differentiation and network connectivity.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
