| Field | Specification |
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| Host | |
| Immunogen | E.coli-derived human TAS2R10 recombinant protein (Position: E16-T307) was used as the immunogen for the TAS2R10 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
| UniProt # |
Overview
TAS2R10 Antibody / Taste receptor type 2 member 10 is a anti-TAS2R10 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Vesicles, Actin filaments, Plasma membrane.
Key elements and design rationale
- Target: TAS2R10
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IF, IHC, WB
Biological background
TAS2R10 is a seven-transmembrane receptor that binds structurally diverse bitter ligands and activates intracellular signaling via gustducin or Galpha subunits. Ligand binding leads to activation of phospholipase C beta 2 (PLCB2), production of inositol trisphosphate (IP3), and release of calcium from intracellular stores. This signaling cascade triggers neuronal depolarization and perception of bitterness. In non-gustatory tissues, TAS2R10 signaling influences ciliary motility, bronchodilation, and antimicrobial peptide secretion. Known ligands include denatonium benzoate, quinine, and brucine.
Structurally, TAS2R10 contains seven alpha-helical transmembrane domains, three extracellular loops for ligand recognition, and a cytoplasmic tail that interacts with G proteins. It belongs to the class A rhodopsin-like GPCR family, which mediates diverse sensory and hormonal signals. TAS2R10 is part of the taste receptor type 2 (T2R) subfamily, which includes approximately 25 functional bitter receptors in humans. Co-localization studies show TAS2R10 present in taste buds, bronchial cilia, and intestinal enteroendocrine cells, reflecting its multifunctional physiological roles.
Functionally, TAS2R10 contributes to gustatory perception as well as airway and gastrointestinal physiology. In airway smooth muscle, activation of TAS2R10 induces relaxation through localized calcium signaling, helping to counteract bronchoconstriction. In the gastrointestinal tract, it modulates hormone release and gut motility. TAS2R10 also contributes to innate immune defense by detecting bacterial metabolites and triggering ciliary clearance mechanisms in respiratory epithelium. During development, TAS2R10 expression appears after taste bud differentiation, coinciding with sensory nerve maturation.
Dysregulation or genetic variation in TAS2R10 may influence taste perception, dietary preferences, and susceptibility to airway diseases. Polymorphisms affecting receptor sensitivity are linked to interindividual differences in bitter compound detection. Pathway associations include GPCR signaling, calcium mobilization, and innate immune responses. In pharmacological research, TAS2R10 is being studied for its potential in bronchodilator and metabolic regulation therapies.
The TAS2R10 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
