| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TBKBP1 recombinant protein (Position: D35-E467) was used as the immunogen for the TBKBP1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TBKBP1 Antibody / TANK-binding kinase 1-binding protein 1 is a anti-TBKBP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: TBKBP1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
TBKBP1 functions as part of a network of adaptor proteins, including NAP1 and TANK, that integrate pattern recognition receptor (PRR) signaling from Toll-like receptors, RIG-I-like receptors, and cytosolic DNA sensors. Upon pathogen detection, TBKBP1 recruits TBK1 to perinuclear regions and promotes its activation via phosphorylation. This process is essential for host defense against viral infection. Beyond immune responses, TBKBP1 also participates in autophagy regulation and may influence NF-kappaB signaling, linking innate immune sensing with cell survival and metabolic adaptation.
The TBKBP1 gene is located on chromosome 17q21.31 and encodes a protein with coiled-coil and leucine zipper domains that mediate multimeric complex formation. Expression is observed in immune tissues, liver, and lung, and can be induced by viral infection or interferon stimulation. Structural studies suggest that TBKBP1 provides a flexible scaffold for recruitment of kinases and transcription factors, enabling precise control of signal propagation. Genetic studies have associated TBKBP1 polymorphisms with autoimmune disorders and susceptibility to chronic inflammation.
Immunohistochemical analysis using TBKBP1 antibody reveals cytoplasmic distribution in macrophages, dendritic cells, and epithelial tissues. The antibody is widely used to study antiviral defense mechanisms, cytokine signaling, and innate immune regulation. TBKBP1 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
