| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TBR1 recombinant protein (Position: K15-S682) was used as the immunogen for the TBR1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TBR1 Antibody / T-box brain protein 1 is a anti-TBR1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TBR1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Functionally, TBR1 antibody identifies a 682-amino-acid nuclear protein that binds T-box DNA consensus sequences to regulate the expression of genes involved in neuronal subtype identity and projection targeting. TBR1 interacts with transcriptional co-regulators such as CASK, FOXP2, and SOX5, forming complexes that guide early cortical neuron differentiation and maturation.
The TBR1 gene is located on chromosome 2q24.2 and is expressed predominantly in postmitotic glutamatergic neurons of the developing and adult cerebral cortex. It functions as a key determinant of cortical layer VI identity and regulates axonal projections to subcortical targets. TBR1 is a downstream effector of the transcription factor PAX6, linking progenitor differentiation to neuronal specialization.
Pathologically, TBR1 mutations have been associated with autism spectrum disorders, intellectual disability, and cortical malformations. Its dysregulation disrupts neuronal migration, synapse formation, and interhemispheric connectivity. Research using TBR1 antibody provides insights into neurodevelopmental biology, transcriptional regulation, and disease mechanisms involving cortical circuitry.
TBR1 antibody is suitable for western blotting, immunofluorescence, and immunohistochemistry to label developing cortical neurons and nuclear transcription factors.
Structurally, T-box brain protein 1 contains a central T-box DNA-binding domain that mediates sequence-specific gene regulation. It acts as both an activator and repressor depending on its interacting partners. This antibody enables researchers to explore TBR1's roles in cortical specification and neural differentiation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
