| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TCIRG1 recombinant protein (Position: Q48-D680) was used as the immunogen for the TCIRG1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TCIRG1 Antibody / T-cell immune regulator 1 is a anti-TCIRG1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: TCIRG1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): IHC, FACS, ELISA
Biological background
TCIRG1 is encoded by the TCIRG1 gene on human chromosome 11q13.2. The protein is approximately 830 amino acids long and represents the a3 isoform of the V-ATPase a subunit. It localizes to the ruffled border membrane of osteoclasts, where it pumps protons into the bone resorption lacuna, dissolving mineralized matrix and enabling bone remodeling. In other cell types, TCIRG1 contributes to lysosomal acidification and vesicle trafficking.
The TCIRG1 antibody detects a 100 kilodalton band by western blot and shows membrane and vesicular staining under immunofluorescence microscopy. Mutations in TCIRG1 cause autosomal recessive osteopetrosis, a disorder characterized by defective bone resorption, increased bone density, and bone marrow failure. In immune cells, TCIRG1 expression regulates antigen processing and presentation by acidifying endolysosomal compartments.
Beyond bone biology, TCIRG1 plays roles in metabolic adaptation and intracellular pH regulation. It interacts with other V-ATPase subunits to control organelle acidification in neurons, hepatocytes, and epithelial cells. Dysregulation of TCIRG1 or V-ATPase activity contributes to cancer cell invasion and multidrug resistance due to altered extracellular acidification.
As a vital component of the proton pump machinery, TCIRG1 links acid-base regulation with immune and skeletal homeostasis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
