| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TCOF1 recombinant protein (Position: M1-K1340) was used as the immunogen for the TCOF1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TCOF1 Antibody / Treacle protein is a anti-TCOF1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, Nucleolar.
Key elements and design rationale
- Target: TCOF1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
Functionally, TCOF1 antibody identifies a 1,488-amino-acid protein localized to the nucleolus, where it interacts with upstream binding factor (UBF) and RNA polymerase I machinery. TCOF1 facilitates 47S pre-rRNA transcription and processing, linking nucleolar activity to cell growth and differentiation. It also participates in DNA damage response by stabilizing nucleolar integrity under stress.
The TCOF1 gene is located on chromosome 5q32-q33.1 and is highly expressed in neural crest-derived and proliferative tissues. Proper TCOF1 function is essential for craniofacial bone and cartilage formation, as neural crest cells depend on efficient ribosome production to sustain rapid proliferation.
Pathologically, haploinsufficiency or mutations in TCOF1 cause Treacher Collins syndrome (TCS), a congenital craniofacial disorder characterized by hypoplasia of facial bones and cleft palate. Defective TCOF1 disrupts ribosome biogenesis, triggering nucleolar stress and apoptosis in neural crest progenitors. Research using TCOF1 antibody supports studies in ribosome assembly, developmental biology, and congenital disease mechanisms.
TCOF1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect nucleolar proteins involved in ribosome production.
Structurally, Treacle protein contains multiple serine-rich and acidic domains that mediate nucleolar localization and protein-protein interactions. This antibody enables analysis of TCOF1's regulatory role in ribosome synthesis and neural crest development.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
