| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TCTN1 recombinant protein (Position: Q118-Y470) was used as the immunogen for the TCTN1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TCTN1 Antibody / Tectonic 1 is a anti-TCTN1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Actin filaments, Primary cilium, Nucleoplasm.
Key elements and design rationale
- Target: TCTN1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Functionally, TCTN1 antibody identifies a 765-amino-acid protein that interacts with other tectonic complex components such as TCTN2, TCTN3, and MKS1. TCTN1 regulates protein trafficking into the cilium and ensures the proper localization of signaling receptors, including those of the Hedgehog pathway. It is indispensable for normal morphogenesis and neural tube patterning.
The TCTN1 gene is located on chromosome 12q24.11 and is expressed in ciliated cells throughout various tissues, including brain, kidney, and retina. Its activity maintains ciliary structure and mediates signal coordination during development and organogenesis.
Pathologically, mutations in TCTN1 lead to ciliopathies such as Joubert and Meckel-Gruber syndromes, which are characterized by developmental defects in the brain, kidneys, and eyes. Dysfunction of TCTN1 disrupts ciliary signaling, resulting in structural malformations and impaired embryonic patterning. Research using TCTN1 antibody supports studies in ciliary biology, developmental signaling, and ciliopathy mechanisms.
TCTN1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect ciliary transition zone proteins.
Structurally, Tectonic-1 contains multiple coiled-coil regions and a C-terminal transmembrane domain that anchor it to the ciliary membrane. This antibody enables investigation of TCTN1's function in ciliogenesis and Hedgehog pathway regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
