| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TDO2 recombinant protein (Position: K16-D406) was used as the immunogen for the TDO2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TDO2 Antibody / Tryptophan 2,3-dioxygenase is a anti-TDO2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: TDO2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Functionally, TDO2 antibody identifies a homotetrameric enzyme that incorporates molecular oxygen into the indole ring of tryptophan. The enzyme is predominantly expressed in liver but is inducible in many tumor types. TDO2 activity shapes the immune microenvironment by depleting tryptophan and generating immunosuppressive kynurenine metabolites. This mechanism contributes to tumor immune evasion and cancer progression.
The TDO2 gene is located on chromosome 4q32.1 and encodes a 406-amino-acid protein with a conserved heme-binding pocket. Its expression is transcriptionally induced by glucocorticoids, stress, and pro-inflammatory cytokines. Elevated TDO2 levels have been reported in hepatocellular carcinoma, glioblastoma, and melanoma, positioning it as a therapeutic target in oncology. TDO2 also regulates neuroactive tryptophan metabolites implicated in mood disorders and neurodegeneration.
Pathologically, upregulation of TDO2 disturbs immune cell proliferation and antigen presentation through depletion of tryptophan and accumulation of kynurenine. Inhibiting TDO2 restores T-cell activity and is an emerging immunotherapeutic strategy complementary to checkpoint blockade. In metabolic studies, altered TDO2 activity links to hepatic inflammation and altered redox homeostasis.
TDO2 antibody is widely applied in cancer immunology, metabolism, and neurobiology research. It is suitable for immunoblotting, immunohistochemistry, and enzyme assays detecting TDO2 expression or localization.
Structurally, tryptophan 2,3-dioxygenase possesses a catalytic heme group coordinated by a proximal histidine. Substrate binding induces conformational changes that enhance dioxygen activation. This antibody supports biochemical and translational studies targeting TDO2 for metabolic modulation and immunotherapy development.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
