| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TDP1 recombinant protein (Position: M1-R499) was used as the immunogen for the TDP1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TDP1 Antibody / Tyrosyl-DNA phosphodiesterase 1 is a anti-TDP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: TDP1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, FACS, ELISA
Biological background
Functionally, TDP1 antibody identifies a 608-amino-acid nuclear enzyme that hydrolyzes the phosphodiester bond linking tyrosine residues of topoisomerase I to the 3? end of DNA. TDP1 also processes other blocked DNA termini generated by oxidative damage or chemotherapeutic agents. Its catalytic mechanism involves two histidine residues acting in a transesterification reaction, forming a transient covalent intermediate. TDP1 cooperates with PARP1 and XRCC1 in the single-strand break repair pathway to restore DNA integrity.
The TDP1 gene is located on chromosome 14q32.11 and is expressed in a broad range of tissues, with highest levels in brain, thymus, and testis. Expression increases in response to DNA damage and genotoxic stress, indicating a role in stress-induced DNA repair. TDP1 activity supports genomic maintenance in neurons, where oxidative DNA damage is frequent.
Pathologically, mutations in TDP1 cause spinocerebellar ataxia with axonal neuropathy (SCAN1), a rare neurodegenerative disorder characterized by progressive motor impairment and neuronal DNA repair deficiency. In cancer, TDP1 confers resistance to topoisomerase I inhibitors such as camptothecin and topotecan by removing drug-stabilized cleavage complexes. Research using TDP1 antibody supports studies in DNA repair, cancer pharmacology, and neurodegeneration.
TDP1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect DNA repair enzymes.
Structurally, Tyrosyl-DNA phosphodiesterase 1 consists of two domains forming an alpha/beta sandwich fold that positions catalytic histidine residues in the active site. The enzyme's flexible loops accommodate diverse DNA substrates, and phosphorylation or SUMOylation modulates its repair activity. This antibody facilitates investigation of TDP1's role in DNA repair, topoisomerase regulation, and neuroprotection.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
