| Field | Specification |
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| Mfr No | |
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| Host | |
| Immunogen | E.coli-derived human TDRD9 recombinant protein (Position: E71-K1339) was used as the immunogen for the TDRD9 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TDRD9 Antibody / Tudor domain-containing protein 9 is a anti-TDRD9 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TDRD9
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, TDRD9 antibody identifies a 1,278-amino-acid protein containing a C-terminal helicase domain and an N-terminal Tudor domain. The Tudor domain recognizes methylated arginine residues on PIWI proteins, while the helicase domain unwinds RNA substrates during piRNA amplification. TDRD9 forms complexes with MIWI2 and other germline-specific proteins to silence retrotransposons through DNA methylation and RNA degradation.
The TDRD9 gene is located on chromosome 14q21.1 and is expressed predominantly in male germ cells during spermatogenesis. It is localized to the cytoplasmic nuage and intermitochondrial cement structures in developing spermatocytes. TDRD9 activity ensures transposon repression and proper germ cell development.
Pathologically, mutations in TDRD9 cause non-obstructive azoospermia and male infertility due to germline arrest and failure of transposon silencing. Dysregulation of TDRD9 or the piRNA pathway may also contribute to germ cell tumors and epigenetic instability. Research using TDRD9 antibody supports studies in germline biology, RNA regulation, and epigenetic silencing.
TDRD9 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect germline helicases and piRNA pathway components.
Structurally, Tudor domain-containing protein 9 contains conserved helicase motifs typical of the DEXD/H-box family and a Tudor domain that mediates protein-RNA interactions. This antibody enables investigation of TDRD9's molecular functions in piRNA biogenesis and transposon repression.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
