| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TERF2IP recombinant protein (Position: M29-K399) was used as the immunogen for the TERF2IP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TERF2IP Antibody is a anti-TERF2IP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: TERF2IP
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, ELISA
Biological background
TERF2IP is encoded by the TERF2IP gene located on human chromosome 16p13.3. The protein is approximately 399 amino acids long and consists of an N-terminal BRCT domain involved in protein interactions and a C-terminal Myb-like DNA-binding domain that anchors it to telomeric DNA via TERF2. TERF2IP localizes to telomeres throughout the cell cycle, forming a stabilizing subcomplex with TERF2 that suppresses activation of the ATM kinase pathway, a major mediator of DNA damage response.
The TERF2IP antibody detects a 45-60 kilodalton band by western blot and shows punctate nuclear foci corresponding to telomere clusters by immunofluorescence. Beyond its telomeric role, TERF2IP functions in transcriptional regulation through the NF-kappaB pathway. In the cytoplasm, it interacts with the IKK complex and influences inflammatory signaling, demonstrating dual nuclear and cytoplasmic roles.
Loss of TERF2IP function results in telomere uncapping, increased DNA damage foci, and cellular senescence. Mutations or mislocalization of TERF2IP have been linked to premature aging syndromes, cancer progression, and metabolic disorders. Its overexpression enhances survival of tumor cells under stress by repressing telomeric DNA damage checkpoints and promoting NF-kappaB-mediated gene expression.
Because TERF2IP integrates telomere maintenance with stress and inflammatory signaling, it serves as an important marker for genomic stability and cancer biology research.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
