| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TFAP4 recombinant protein (Position: M1-K299) was used as the immunogen for the TFAP4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TFAP4 Antibody / Transcription factor AP-4 is a anti-TFAP4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear.
Key elements and design rationale
- Target: TFAP4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
Functionally, TFAP4 antibody identifies a 338-amino-acid nuclear transcription factor that acts as both an activator and repressor of transcription. TFAP4 dimerizes through its leucine zipper domain and binds to specific promoter sequences, influencing the expression of genes controlling cell cycle progression, epithelial-to-mesenchymal transition (EMT), and differentiation. It regulates genes such as MYC, CDKN1A (p21), and CCND1, linking growth control to transcriptional responses.
The TFAP4 gene is located on chromosome 16p13.3 and is broadly expressed in epithelial and immune cells. TFAP4 functions downstream of MYC as a key mediator of its transcriptional program. In oncogenesis, TFAP4 promotes proliferation, invasion, and metastasis by activating EMT-related transcription factors such as SNAIL and repressing cell adhesion molecules like E-cadherin. Conversely, under stress conditions, TFAP4 can trigger apoptotic pathways through p53-dependent transcriptional activation.
TFAP4 also participates in development by regulating neuronal and epithelial differentiation. Its activity is modulated by post-translational modifications including phosphorylation and acetylation, which influence its DNA-binding affinity and cofactor recruitment. Dysregulation of TFAP4 expression has been linked to various cancers, including colorectal, breast, and gastric cancer, where it drives metastasis and stem-like phenotypes.
TFAP4 antibody is widely used in transcriptional regulation, cancer biology, and developmental studies. It is suitable for western blotting, immunohistochemistry, and chromatin immunoprecipitation to detect TFAP4 protein levels and promoter occupancy. This antibody supports investigations into MYC signaling, cell cycle regulation, and transcriptional reprogramming. In oncology, TFAP4 detection provides insights into tumor progression and epithelial plasticity.
Structurally, TFAP4 contains a bHLH-LZ domain for DNA binding and dimerization, along with transactivation and repression regions that recruit chromatin-modifying complexes.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
