| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TFDP3 recombinant protein (Position: M1-E305) was used as the immunogen for the TFDP3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TFDP3 Antibody / Transcription factor Dp family member 3 is a anti-TFDP3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA with listed reactivity in Human. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: TFDP3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, ELISA
Biological background
TFDP3 is encoded by the TFDP3 gene located on human chromosome Xq26.2. The protein consists of a conserved DNA-binding domain and a dimerization region that facilitates complex formation with E2F1. This heterodimerization blocks the transcriptional activation of E2F target genes involved in DNA synthesis and cell cycle progression. As a result, TFDP3 contributes to cell cycle arrest and resistance to apoptosis, especially under stress or DNA damage conditions.
The TFDP3 antibody is used to detect the 45-47 kDa nuclear protein in various cell lines and tissues. Immunofluorescence reveals nuclear localization consistent with its function as a transcriptional regulator. TFDP3 is predominantly expressed in testis, certain stem cells, and a subset of cancer tissues. Its overexpression has been reported in hepatocellular carcinoma, lung adenocarcinoma, and breast cancer, where it promotes survival by suppressing E2F1-induced apoptosis. Mechanistically, TFDP3 interferes with E2F1's ability to induce p53-independent cell death, thereby facilitating oncogenic proliferation.
Beyond cell cycle regulation, TFDP3 participates in differentiation processes and developmental gene expression control. It is also implicated in DNA repair regulation and stress-induced transcriptional reprogramming. Studies have shown that silencing TFDP3 enhances the cytotoxic effects of chemotherapeutic agents, making it a potential therapeutic target.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
