| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TFEC recombinant protein (Position: Q27-D311) was used as the immunogen for the TFEC antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TFEC Antibody / Transcription factor EC is a anti-TFEC Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TFEC
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
TFEC is encoded by the TFEC gene located on human chromosome 7q31.2. The protein is approximately 38 kilodaltons and contains a conserved DNA-binding bHLH-LZ domain that mediates binding to E-box sequences in gene promoters. TFEC forms homodimers or heterodimers with other MiT family members, allowing versatile regulation of target gene networks. It localizes to the nucleus and is activated by phosphorylation under stress or inflammatory conditions.
The TFEC antibody detects a 38 kilodalton protein by western blot and demonstrates nuclear staining in macrophages and osteoclast precursors. TFEC regulates transcription of lysosomal and autophagy-related genes, participating in cellular adaptation to nutrient deprivation. It also modulates inflammatory gene expression through cooperation with PU.1 and other macrophage-specific transcription factors, promoting cytokine and chemokine production.
In melanocytes, TFEC contributes to pigment cell differentiation and interacts with MITF to balance melanogenic gene expression. Dysregulation of TFEC has been implicated in cancer, where fusion genes involving TFEC drive aberrant transcriptional programs. Altered expression is also associated with inflammatory and metabolic disorders due to disrupted lysosomal signaling and autophagic control.
TFEC integrates environmental and metabolic signals to maintain homeostasis in immune and pigment cells.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
