| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human BIGH3/TGFBI recombinant protein (Position: K27-H683) was used as the immunogen for the TGFBI antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TGFBI Antibody / Transforming growth factor-beta-induced protein ig-h3 is a anti-TGFBI Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TGFBI
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, FACS, ELISA
Biological background
Transforming growth factor-beta-induced protein ig-h3 is secreted into the extracellular space, where it binds integrins such as alphavbeta3 and alpha3beta1 to regulate adhesion and cell-matrix communication. It is widely expressed in corneal, epithelial, and connective tissues. The TGFBI antibody allows visualization of its distribution and quantification under TGF-beta stimulation, providing insight into how this protein orchestrates matrix remodeling. Its RGD motif facilitates integrin binding, influencing cell attachment, migration, and differentiation.
Mutations in the TGFBI gene cause a spectrum of hereditary corneal dystrophies, including granular, lattice, and Avellino types, characterized by amyloid or hyaline deposits in the cornea. The TGFBI antibody supports studies into these pathologies, enabling detection of protein accumulation and abnormal deposition patterns. By tracking misfolded Transforming growth factor-beta-induced protein ig-h3, researchers can better understand disease progression and potential therapeutic interventions aimed at preventing protein aggregation.
Beyond ocular disease, TGFBI is implicated in cancer, fibrosis, and tissue regeneration. It may function as either a tumor suppressor or promoter depending on context, influencing epithelial-to-mesenchymal transition (EMT) and extracellular matrix stiffness. The TGFBI antibody supports exploration of these dual roles by enabling quantification of expression levels in tumor microenvironments and fibrotic tissues. TGFBI overexpression is associated with enhanced metastasis in certain cancers, making it a potential biomarker for disease progression.
The TGFBI antibody performs well in western blotting, immunohistochemistry, and immunofluorescence, showing distinct extracellular and pericellular staining consistent with matrix localization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
