| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TGIF2 recombinant protein (Position: M1-Q237) was used as the immunogen for the TGIF2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TGIF2 Antibody / TG-interacting factor 2 is a anti-TGIF2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA with listed reactivity in Human. Reported localization: Nucleus, Centrosome.
Key elements and design rationale
- Target: TGIF2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, IP, ELISA
Biological background
Functionally, TGIF2 antibody identifies a 237-amino-acid protein localized in the nucleus, where it binds DNA at consensus homeobox sites and interacts with transcriptional repressors such as CtBP and HDACs. TGIF2 represses TGF-beta target genes by blocking Smad binding to promoters, fine-tuning cellular differentiation, proliferation, and morphogenesis during embryonic development.
The TGIF2 gene is located on chromosome 20q11.2 and is expressed in embryonic tissues and adult organs including brain, kidney, and testis. TGIF2 acts as a developmental regulator controlling anterior-posterior patterning and organ formation through transcriptional repression mechanisms.
Pathologically, TGIF2 overexpression has been observed in several cancers, including ovarian and gastric tumors, where it promotes cell proliferation and epithelial-mesenchymal transition. Gene duplications or deletions involving TGIF2 have been associated with holoprosencephaly and craniofacial malformations. Research using TGIF2 antibody supports studies in transcriptional repression, developmental biology, and oncogenesis.
TGIF2 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect homeodomain transcription factors.
Structurally, TG-interacting factor 2 contains a homeobox DNA-binding domain and a CtBP-binding motif that mediate its repressor activity. This antibody aids in analyzing TGIF2's role in gene regulation, morphogenesis, and signaling cross-talk.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
