| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TGM4 recombinant protein (Position: M1-K684) was used as the immunogen for the TGM4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TGM4 Antibody / Transglutaminase 4 is a anti-TGM4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TGM4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, TGM4 antibody identifies a 684-amino-acid secreted and membrane-associated protein primarily expressed in the prostate and other reproductive tissues. TGM4 cross-links seminal plasma proteins, forming insoluble networks that contribute to semen coagulation and fertility. It may also participate in epithelial barrier formation and immune defense in the urogenital tract.
The TGM4 gene is located on chromosome 3p21.33 and is expressed in male reproductive tissues and epithelial surfaces. TGM4 activity is regulated by calcium ions and thiol-reactive compounds, influencing enzyme conformation and catalytic activity.
Pathologically, altered TGM4 expression has been associated with prostate cancer and infertility. Decreased expression correlates with impaired seminal coagulation, while increased levels are observed in prostate tumors and serve as potential diagnostic biomarkers. Research using TGM4 antibody supports studies in enzymology, reproductive biology, and oncology.
TGM4 antibody is validated for western blotting, immunohistochemistry, and ELISA to detect transglutaminase enzymes.
Structurally, Transglutaminase 4 consists of a catalytic core domain containing the active site cysteine, histidine, and aspartate residues, flanked by beta-sandwich and C-terminal domains that mediate substrate binding. This antibody enables analysis of TGM4’s role in cross-linking reactions and reproductive function.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
