| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TGM7 recombinant protein (Position: E147-D701) was used as the immunogen for the TGM7 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TGM7 Antibody / Transglutaminase 7 is a anti-TGM7 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: TGM7
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, TGM7 participates in the formation of insoluble protein structures within epithelial cells, supporting tissue integrity under mechanical and environmental stress. It crosslinks structural proteins such as keratins, involucrin, and loricrin, enhancing the strength of epithelial layers. Co-localization studies show TGM7 associating with the plasma membrane and cytoskeletal components during terminal differentiation of keratinocytes. The enzyme also contributes to barrier maintenance in the gastrointestinal tract and reproductive system.
Structurally, TGM7 shares conserved catalytic residues with other transglutaminases, including the catalytic triad of cysteine, histidine, and aspartate. It contains a core transamidase domain flanked by beta-sandwich and beta-barrel domains that facilitate substrate recognition and enzymatic activity. TGM7 is part of the transglutaminase enzyme family, which includes TGM1-6 and Factor XIIIa, all of which catalyze similar crosslinking reactions. Calcium binding activates TGM7, promoting conformational changes that expose the catalytic site for substrate processing.
TGM7 plays roles beyond structural reinforcement, including regulation of cell adhesion, apoptosis, and immune responses. It has been shown to modulate integrin signaling and extracellular matrix interactions. Dysregulation of TGM7 expression is linked to skin disorders, inflammatory bowel disease, and cancer. Overexpression has been observed in colorectal and pancreatic tumors, where TGM7 may promote cell migration and invasion through cytoskeletal remodeling. Pathway involvement includes keratinocyte differentiation, cell adhesion, and calcium signaling.
Tissue-specific studies indicate that TGM7 expression is high in differentiated epithelial cells and germ cells, reflecting its contribution to terminal differentiation and reproductive tissue function. During embryonic development, it supports epithelial morphogenesis and barrier establishment. Its enzymatic crosslinking activity provides stability to tissues exposed to environmental stressors such as mechanical abrasion and chemical insult.
Immunohistochemical staining using TGM7 antibody shows cytoplasmic and membrane localization in epithelial tissues and testis. The TGM7 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
