| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TGN46/TGOLN2 recombinant protein (Position: E319-K436) was used as the immunogen for the TGOLN2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TGOLN2 Antibody / Trans-Golgi network integral membrane protein 2 is a anti-TGOLN2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: TGOLN2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Trans-Golgi network integral membrane protein 2 cycles between the TGN and plasma membrane via clathrin-coated vesicles, controlling cargo retrieval and receptor recycling. Its luminal domain carries O-linked glycosylation sites that modulate trafficking efficiency. The TGOLN2 antibody allows visualization of this dynamic localization pattern, offering a reliable marker for confocal microscopy and subcellular fractionation studies. Its expression is ubiquitous in secretory cells, particularly in liver, pancreas, and neurons.
TGN46 functions as a sorting receptor for a subset of secreted and membrane proteins, ensuring their correct targeting to the cell surface or lysosomes. The TGOLN2 antibody supports mechanistic research into Golgi transport, revealing how membrane trafficking contributes to cellular polarity and secretion. Through its cytoplasmic tail, TGN46 interacts with adaptor protein complexes (AP-1 and GGA), linking it to clathrin-mediated sorting pathways.
Aberrant Golgi organization is a hallmark of many diseases, including neurodegenerative disorders and cancers. The TGOLN2 antibody is widely used as a Golgi marker in pathology and cell-biology research, helping identify structural changes associated with altered vesicular transport. Changes in TGN46 distribution can reflect stress-induced fragmentation or disrupted trafficking in diseased cells.
The TGOLN2 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, yielding strong perinuclear staining that defines Golgi morphology.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
