| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | Recombinant full-length human Zinc finger and BTB domain-containing protein 7B protein was used as the immunogen for the Th-Pok antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TH-POK (T-helper-inducing POZ/Kr ppel-like factor), also known as zinc finger protein 67 (ZFP67), zinc finger and BTB domain-containing protein 7B or Kr ppel-related zinc finger protein cKrox, is a 539 amino acid protein that contains one BTB (POZ) domain and four C2H2-type zinc fingers. Localized to the nucleus, TH-POK functions primarily as a key regulator of lineage commitment of immature T-cell precursors. Specifically, the presence of TH-POK directs positively selected thymocytes to the CD4 lineage, whereas its absence causes default development to the CD8 lineage. TH-POK also functions as a transcriptional repressor of various other genes, such as COL1A1, COL1A2 and Fibronectin.
This anti-ZBTB7B antibody is supplied as Purified (Mouse, Monoclonal (mouse origin), clone PCRP-ZBTB7B-1B6, Mouse IgG2b, Unconjugated) and is designed to support common target-detection workflows after the on-page specifications.
Key elements and design rationale
- Target: ZBTB7B
- Format: Purified
- Localization: Nucleus
- Species reactivity: Human
- Applications (listed): FACS, IF, WB
- Conjugate: Unconjugated
- Clone and antibody class: Monoclonal (mouse origin), clone PCRP-ZBTB7B-1B6, Mouse IgG2b
Because antibody performance can depend on epitope context, sample preparation, and biological state, interpret signals using appropriate controls and orthogonal evidence when possible.
Biological background
ZBTB7B is referenced in public gene/protein resources (e.g., UniProt and NCBI Gene), which provide curated names/synonyms, protein features, and pathway context. When designing assays, consider potential isoforms, post-translational modifications, and cell-type specific expression that may influence observed signal.
Research relevance and current trends
- Profiling ZBTB7B expression across model systems, perturbations, and time points to support mechanistic hypotheses.
- Combining antibody-based detection with multi-omics or imaging readouts to link ZBTB7B signal with phenotype.
- Using well-matched controls (isotype controls, genetic perturbations, or independent reagents) to strengthen interpretation of target-associated signal.
Common research applications
- FACS
- IF
- WB
Use the listed applications as a starting point and tailor experimental design to your sample type and readout requirements.
Notes for experimental interpretation
- Specificity considerations: closely related family members, isoforms, or PTMs can affect apparent specificity; confirm with independent approaches when critical.
- Controls: include negative controls and, when feasible, genetic or pharmacologic perturbations to support target attribution in your system.
- Species and sample context: differences in sequence, expression, fixation, or extraction conditions can change signal behavior across models.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
