| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human THEM6 recombinant protein (Position: Y23-Q208) was used as the immunogen for the THEM6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
THEM6 Antibody / Thioesterase superfamily member 6 is a anti-THEM6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: THEM6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, IP, FACS, ELISA
Biological background
Functionally, THEM6 antibody identifies a 247-amino-acid protein localized primarily in the endoplasmic reticulum (ER). THEM6 catalyzes the hydrolysis of long-chain acyl-CoA molecules, controlling intracellular levels of activated fatty acids and maintaining the balance between lipid synthesis and degradation. By regulating acyl-CoA and free fatty acid pools, THEM6 influences energy metabolism, membrane composition, and lipid signaling cascades.
The THEM6 gene is located on chromosome 1p36.33 and encodes an enzyme expressed in metabolically active tissues, including liver, adipose tissue, and skeletal muscle. THEM6 contains a hotdog-fold thioesterase domain characteristic of enzymes that process acyl-CoA intermediates. Its activity is critical for preventing the accumulation of toxic acyl-CoA derivatives that can impair mitochondrial function and oxidative metabolism. THEM6 also participates in lipid remodeling pathways that maintain ER membrane homeostasis.
In cellular metabolism, THEM6 acts as a regulator of lipid droplet dynamics and fatty acid oxidation. It modulates the availability of CoA for beta-oxidation and phospholipid biosynthesis, indirectly influencing mitochondrial energy production. Dysregulation of THEM6 expression has been linked to metabolic disorders, including fatty liver disease and obesity. Emerging evidence suggests a role for THEM6 in cancer cell metabolism, where enhanced lipid turnover supports rapid proliferation and membrane biogenesis.
THEM6 antibody is widely used in lipid metabolism, enzymology, and metabolic disease research. It is suitable for western blotting, immunofluorescence, and subcellular localization studies to detect THEM6 expression in cellular and tissue samples. This antibody supports studies of acyl-CoA metabolism, lipid homeostasis, and ER stress responses. In metabolic and cancer research, THEM6 detection provides insights into how cells reprogram lipid utilization under stress or nutrient limitation.
Structurally, THEM6 contains a conserved thioesterase catalytic triad composed of serine, histidine, and aspartate residues, mediating acyl-CoA hydrolysis. Its ER localization is maintained through a C-terminal retention signal, and its expression may be regulated by nutrient and hormonal cues.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
