| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human THYN1 recombinant protein (Position: M1-S225) was used as the immunogen for the THYN1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
THYN1 Antibody / Thymocyte nuclear protein 1 is a anti-THYN1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear.
Key elements and design rationale
- Target: THYN1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, IP, FACS, ELISA
Biological background
Structurally, Thymocyte nuclear protein 1 is a 225-amino-acid protein of approximately 26 kilodaltons that localizes to the nucleus. It contains multiple lysine and arginine-rich regions that facilitate DNA interaction and chromatin association, as well as nuclear localization sequences that ensure retention within the nucleus. Its precise molecular function remains under study, but evidence suggests that THYN1 participates in apoptotic chromatin condensation and transcriptional control during cell stress and differentiation.
The THYN1 antibody is widely used in immunology, apoptosis, and hematopoiesis research to study nuclear regulation of T-cell development and programmed cell death. Western blot analysis detects a band at approximately 28 kilodaltons corresponding to THYN1, while immunofluorescence demonstrates nuclear punctate staining consistent with chromatin association. This antibody is a valuable tool for understanding nuclear protein dynamics in immune cell differentiation and apoptosis signaling.
Functionally, THYN1 has been linked to regulation of the apoptotic pathway through interactions with caspase-dependent DNA fragmentation processes. Its expression is highest in thymocytes undergoing negative selection, suggesting a role in eliminating autoreactive T-cells. Beyond immune regulation, THYN1 may contribute to chromatin remodeling and transcriptional repression during stress-induced cell cycle arrest. Dysregulation of THYN1 has been associated with cancer cell survival and abnormal immune function, underscoring its potential role in tumor immunology and apoptosis resistance. The THYN1 antibody supports investigations into nuclear apoptosis mechanisms and T-cell homeostasis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
