| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human TIM50 was used as the immunogen for the TIM50 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TIM50 Antibody / Translocase of inner mitochondrial membrane 50 is a anti-TIM50 Rabbit antibody Recombinant Rabbit Monoclonal clone 32T72 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TIM50
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32T72, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
As a receptor subunit of the TIM23 complex, TIM50 binds to presequences on precursor proteins as they enter the intermembrane space. Research using TIM50 antibody has shown that this interaction is required for recognition and transfer of proteins from the TOM complex in the outer membrane to the TIM23 channel in the inner membrane. TIM50 also acts as a gatekeeper, regulating the opening and closing of the translocation channel in response to membrane potential and precursor signals. This makes TIM50 indispensable for mitochondrial protein import.
Dysfunction of TIMM50 is linked to mitochondrial disease. Mutations in the TIMM50 gene cause combined oxidative phosphorylation deficiency, characterized by developmental delay, seizures, and lactic acidosis. Studies with TIM50 antibody have confirmed that mutations reduce protein import efficiency and disrupt mitochondrial homeostasis. Because proper protein import is essential for assembly of respiratory chain complexes, TIM50 deficiency leads to impaired energy metabolism and neuromuscular symptoms.
Beyond rare genetic syndromes, TIM50 has been implicated in cancer and neurodegeneration. Research with TIM50 antibody has demonstrated altered expression in tumors, where mitochondrial remodeling supports rapid growth. In neurons, reduced TIM50 activity contributes to mitochondrial fragmentation and synaptic dysfunction. These findings highlight its broader significance beyond classical mitochondrial import pathways.
TIM50 antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects TIM50 expression in mitochondria rich tissues, immunohistochemistry localizes it to muscle and brain, and immunofluorescence confirms inner membrane localization with colocalization to mitochondrial markers. These applications make TIM50 antibody essential for studying mitochondrial protein transport.
By supplying validated TIM50 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
