| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human TIMM22 was used as the immunogen for the TIMM22 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
| UniProt # |
Overview
TIMM22 Antibody / Translocase of inner mitochondrial membrane 22 is a anti-TIMM22 Rabbit antibody Recombinant Rabbit Monoclonal clone 31T40 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TIMM22
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31T40, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
TIMM22 antibody is widely applied in mitochondrial biology and protein trafficking research. By detecting TIMM22, scientists can study how mitochondrial import machinery regulates oxidative phosphorylation and cellular energy production. Defects in TIMM22 function lead to impaired assembly of carrier proteins such as ADP/ATP translocases, disrupting mitochondrial function and cellular homeostasis.
Western blot assays detect TIMM22 protein in mitochondrial fractions, while immunohistochemistry highlights expression in tissues with high metabolic demand such as muscle, heart, and brain. Immunofluorescence reveals punctate mitochondrial staining, consistent with its localization in the inner membrane. These methods allow precise analysis of mitochondrial import processes.
Mutations in TIMM22 and disruption of mitochondrial protein import are linked to metabolic disorders, neurodegeneration, and cardiomyopathy. By applying TIMM22 antibody, researchers can investigate how defects in import machinery contribute to disease and explore therapeutic strategies to restore mitochondrial function.
TIMM22 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
