| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A portion of amino acids 130-158 from the human protein was used as the immunogen for this TIMM23 antibody. |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TIMM23 Antibody / Tim23 is an antibody targeting TIMM23, raised in Rabbit for protein detection and localization studies where these specifications are required.
Key elements and design rationale
- Target: TIMM23.
- Antibody identity: Polyclonal (rabbit origin); Rabbit Ig.
- Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
- Format: Antigen affinity purified.
- Species reactivity: Human.
- Listed applications: WB (refer to on-page specifications for application-specific guidance).
Biological background
TIMM23, or Translocase of the Inner Membrane 23, is a protein that is found in the inner membrane of the mitochondria. Mitochondria are known as the powerhouses of our cells, producing the energy needed to fuel all of our bodily functions. TIMM23 acts as a gateway, helping to transport proteins into the mitochondria where they can carry out their essential functions. One of the most fascinating aspects of TIMM23 is its ability to recognize and transport proteins with a specific signal sequence. This ensures that only the necessary proteins make their way into the mitochondria, helping to maintain the efficiency and health of the cell. Studies have shown that mutations in the gene encoding TIMM23 can result in mitochondrial dysfunction, leading to a variety of health issues including metabolic disorders and neurodegenerative diseases.
Research relevance and current trends
- Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
- Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
- Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.
Common research applications
- Western blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.
Notes for experimental interpretation
- Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
- Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
- Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
- Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
