| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TIMM8B recombinant protein (Position: M1-Q83) was used as the immunogen for the TIMM8B antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TIMM8B Antibody / Tim8 B is a anti-TIMM8B Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TIMM8B
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Structurally, TIMM8B contains conserved twin CX3C motifs that coordinate zinc ions, stabilizing its folding and interaction with partner subunits. Within the IMS, TIMM8B associates with TIMM13 to form a 70 kilodalton heterohexameric complex. This assembly binds and shuttles hydrophobic transmembrane segments of carrier proteins, preventing their aggregation during translocation. Loss or dysfunction of TIMM8B disrupts mitochondrial protein import, leading to defects in oxidative phosphorylation and metabolic balance. While TIMM8A mutations cause Mohr-Tranebjaerg syndrome, TIMM8B plays a complementary and partially compensatory role in mitochondrial maintenance.
The TIMM8B antibody is widely used in cell biology, mitochondrial, and neurodegeneration research to study protein import mechanisms and mitochondrial function. Western blot analysis identifies a 10 kilodalton band corresponding to TIMM8B, while immunofluorescence reveals punctate mitochondrial localization that colocalizes with markers such as TOM20 and COX IV. This antibody enables precise examination of mitochondrial translocase assembly and protein trafficking. Because mitochondrial dysfunction contributes to neurodegenerative disorders, TIMM8B detection provides valuable insights into pathophysiology and energy metabolism.
Beyond its role in import, TIMM8B contributes to mitochondrial quality control and stress response by maintaining the stability of the carrier import pathway. It supports the biogenesis of metabolite transporters such as ANT, MPC, and phosphate carriers. The TIMM8B antibody supports detailed analysis of these pathways and is validated by
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
