| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TIMM9 recombinant protein (Position: M1-R89) was used as the immunogen for the TIMM9 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TIMM9 Antibody / Tim9 is a anti-TIMM9 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat. Reported localization: Cytoplasm (Mitochondria).
Key elements and design rationale
- Target: TIMM9
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): IHC, IF, FACS, ELISA
Biological background
Structurally, TIMM9 is an 89-amino-acid protein of approximately 10 kilodaltons containing twin CX3C motifs that coordinate zinc ions to stabilize its characteristic hexameric structure. Within the intermembrane space, TIMM9 forms a heterohexameric complex with TIMM10 or TIMM10B, providing a soluble chaperone system that transfers imported precursors from the TOM complex (translocase of the outer membrane) to the TIM22 complex for membrane insertion.
The TIMM9 antibody is widely used in mitochondrial import, bioenergetics, and protein trafficking research to study mitochondrial assembly, carrier translocation, and protein folding. Western blot analysis detects a 10 kilodalton band corresponding to TIMM9, while immunofluorescence demonstrates mitochondrial localization colocalizing with markers such as TOM20 and COX IV. This antibody is valuable for analyzing mitochondrial protein import efficiency and intermembrane space chaperone dynamics.
Functionally, TIMM9 is essential for the import of metabolite carrier proteins, including members of the ADP/ATP translocase and phosphate carrier families. Loss of TIMM9 disrupts carrier protein biogenesis, resulting in impaired mitochondrial respiration and metabolic deficiency. Defects in TIMM9 expression or complex formation have been implicated in mitochondrial encephalopathies and neurodegenerative diseases characterized by energy failure and oxidative stress. The TIMM9 antibody provides a sensitive reagent for evaluating mitochondrial import machinery integrity and studying molecular chaperone systems that maintain organelle function.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
