| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human DAK/TKFC recombinant protein (Position: D15-E538) was used as the immunogen for the TKFC antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TKFC Antibody / Triokinase/FMN cyclase is a anti-TKFC Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: TKFC
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, FACS, ELISA
Biological background
TKFC is encoded by the TKFC gene located on human chromosome 17p13.1. The protein is approximately 585 amino acids long and localizes to the cytoplasm, particularly in liver and kidney tissues, where it supports energy metabolism and flavin cofactor recycling. TKFC functions as a bifunctional enzyme with separate kinase and cyclase domains, ensuring efficient coordination between sugar phosphorylation and FMN homeostasis.
The TKFC antibody detects a 63 kilodalton protein by western blot and shows cytoplasmic staining under immunofluorescence microscopy. TKFC participates in glycerol metabolism by converting trioses into intermediates of glycolysis and gluconeogenesis, thereby contributing to glucose homeostasis. Its FMN cyclase activity recycles flavin cofactors that support oxidative enzymes and mitochondrial redox balance.
Defects in TKFC activity lead to impaired energy metabolism, elevated triose intermediates, and disturbances in flavin homeostasis. Although rare, TKFC deficiency has been linked to metabolic acidosis and hepatic dysfunction. Elevated TKFC expression in tumors reflects metabolic adaptation to high glycolytic flux and enhanced redox cycling, supporting cell proliferation under hypoxia.
By connecting carbohydrate utilization and flavin metabolism, TKFC provides a unique window into cellular bioenergetics and redox control.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
