| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TMC6 recombinant protein (Position: M1-H772) was used as the immunogen for the TMC6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TMC6 Antibody / Transmembrane channel-like protein 6 is a anti-TMC6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nucleus membrane, Golgi.
Key elements and design rationale
- Target: TMC6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, FACS, ELISA
Biological background
Transmembrane channel-like protein 6 is localized mainly to the endoplasmic reticulum and nuclear envelope, where it contributes to the regulation of zinc-dependent transcription factors. The TMC6 antibody supports localization and expression analyses that reveal how zinc flux affects keratinocyte differentiation and immune signaling. Through its partnership with TMC8, it restricts viral replication, particularly for human papillomaviruses (HPVs), by maintaining proper zinc balance within the nucleus.
Mutations in TMC6 cause Epidermodysplasia verruciformis (EV), a rare genodermatosis characterized by chronic HPV infection and increased skin cancer risk. The TMC6 antibody supports studies of this disease by enabling detection of protein loss or mislocalization in affected tissues. Lack of functional Transmembrane channel-like protein 6 disrupts zinc homeostasis, leading to impaired immune defense against HPV and uncontrolled keratinocyte proliferation. This discovery has highlighted TMC6 as a crucial host factor for viral resistance in epithelial tissues.
Beyond antiviral defense, Transmembrane channel-like protein 6 influences immune cell signaling and skin barrier function. The TMC6 antibody aids in quantifying these effects, revealing how altered zinc regulation impacts inflammation and epidermal differentiation. Its expression is highest in stratified epithelia but may also occur in immune cells and other tissues involved in zinc transport and immune surveillance.
The TMC6 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence, providing distinct perinuclear staining consistent with endoplasmic reticulum localization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
